About me
Hello!!! I am a fourth-year Ph.D. scholar in the Cancer Biology Lab, Department of Bioscience and Bioengineering, Indian Institute of Technology (IIT), Guwahati under the supervision of Prof. Ajaikumar B. Kunnumakkara. My primary interest lies in exploring the mechanistic cues of tumor pathophysiology in the early stage of transformation. I believe that cancer cells are both static and dynamic in the temporal planes, and intervening at the early stage will help circumvent the disease. The discovery of microRNAs has brought a paradigm shift in biological research, making it possible for us to focus on the molecular aspects of regulation in disease etiology. My research goals are to develop novel site-specific and effective treatment regimens using non-coding RNAs.
I previously interned at the CSIR-Centre for Cellular and Molecular Biology (CCMB), India, centered around studying the mechanism of initiation and progression of colorectal cancer by using transgenic murine models. I was also a part of the team to understand the effect of DNA damaging drugs on anomalous EMT activation in the early stage of drug treatment, leading to drug resistance. The other projects I had worked on focused on the suppression of breast and colon cancer autochthonous models by using RNA interference and Nanotechnology. After completing my graduation, I joined CCMB as a project trainee to develop and validate the biomarkers for different sub-types (benign, borderline, fibro adenocarcinoma, malignant) of the phyllode tumors using global proteomic analysis, IHCs, and IFs. Apart from this project, I was also working on validating plausible microRNAs for breast cancer diagnosis.
Education
2017
Bachelors of Technolgy (BTech)
Specialization- Biotechnology
Vellore Institute of Technology (VIT), Vellore
Grades- 9.32/ 10
2019
Master's of Technolgy (MTech)
Specialization- Biotechnology
Maulana Azad National Institute of Technology (NIT), Bhopal
Grades- 9.58/ 10
2020
Pursuing Doctor of Philosophy (DPhil)
Indian Institute of Technology (IIT) Guwahati
Experience
2016-2017
Center for Biomaterials, Cellular and Molecular Thernaostic, VIT University
Undergraduate Dissertation Research
Probable role of non-extracellular vesicles in colorectal cancer metastasis to kidney: an in vitro cell line-based study and image analysis
2018-2019
CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad
Graduate Dissertation Research
Genotyping, Characterization and Pre-clinical Studies of transgenic murine models of Colorectal Cancer
Identification and Characterization of biomarkers for different sub-types (benign, borderline, fibro adenocarcinoma,
malignant) present in phyllode tumors
2019-2020
CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad
Project Trainee
Laboratory Skills
In vivo Techniques
Mice Handling, Breeding, Injections- Intraperitoneal, Intratumoral, Oral guvaging, Cardiac Perfusion,
Anesthetize, Histology, Tissue Processing, Sectioning
Immunological Assays
Immunoblots, ELISA, Immunocytochemistry, Immunohistochemistry, Rocket Electrophoresis
Animal Cell Culture
Subculturing, MTT assay, Invasion assay, Scratch assay, FACS, Colony formation assay, Transfection
Microbial Techniques
Plating, Streaking, Transformation, Cloning, Staining, Restriction Digestion
Molecular Biology Techniques
DNA, RNA and Protein Isolation, Plasmid Isolation, PCR, RT-PCR, Agarose Gel Electrophoresis
Computational Techniques
RNA seq analysis, Primer Design, BioEdit, ClustalW, MEGA6, Phylognetics, Autodock, miRNA Target Predcition
Conference Presenations
Feburary 2023
December 2021
March 2019
October 2016
Poster Presentation | Prime Minister's Research Fellows Annual Symposium 2023, Indian Institute of Technology (IIT) Madras
Development of miRNA mimics targeting Tumor necrosis factor-α induced protein eight (TNFAIP8/TIPE) in oral cancer
Oral Presentation | 2nd International Conference on Cancer Metastasis, Austria
Combinatorial therapy using RNAi and curcumin nano- architectures regress tumors in breast and colon cancer models
Oral and Poster Presentation | International Conference on Advances in Chemical Biology and Biologics (ICACB 2019), CSIR- INDIAN INSTITUTE OF CHEMICAL TECHNOLOGY, Hyderabad
Activation of Epithelial-Mesenchymal Transition (EMT) during early drug response inhibits apoptotic progression by stimulating survival pathways in the colon carcinoma model
Poster Presentation | International Conference on Nanoscience and Nanotechnology (ICNAN’16), VELLORE INSTITUTE OF TECHNOLOGY (VIT), Vellore
Use of Membrane filter for isolation of Nanoscopic Extracellular Vesicles from cultured A549 lung cancer cell : A feasibility study
Honours and Awards
2021: ”Certificate of Appreciation” by European Accreditation Council for Continuing Medical Education (EACCME®) for presentation at 2nd International Conference on Cancer Metastasis, Austria
2021: Recipient of Prime Minister's Research Fellow (PMRF) for carrying out the vision of research and development through innovation, Ministry of Education (MOE), Govt. of India
2020: ”Certificate of Appreciation" for contribution as an “Instructor” in the Winter Research Observership Programme for Medical Students (MBBS) at CSIR- Centre for Cellular and Molecular Biology, Hyderabad under
CSIR Integrated Skill Initiative
2019: Best Oral Presentation in International Conference on Advances in Chemical Biology and Biologics (ICACB 2019), by Royal Society of Chemistry, CSIR-Indian Institute of Chemical Technology, Hyderabad
2019: Merit Scholar, First Rank in Department of Biological Science and Engineering, MANIT, Bhopal
2019: Recipient of GATE-MHRD fellowship from the Ministry of Human Resource Development, Govt. of India
2014: Merit Certificate, Best academic performance in Vellore Institute of Technology (VIT)
Referees
Ajaikumar B Kunnumakkara
Professor, Indian Instittute of Technology (IIT) Guwahati
Lekha Dinesh Kumar
Senior Principal Scientist, CSIR- Centre for Cellular and Molecular Biology, Hyderabad
Anindya Goswami
Senior Principal Scientist, CSIR- Indian Institute of Integrative Medicine, Jammu
Hitesh Kulhari
Assistant Professor and DST INSPIRE Faculty, Central University of Gujarat, Gandhinagar