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CV: Welcome
CV: Welcome

About me 

Hello!!! I am a fourth-year Ph.D. scholar in the Cancer Biology Lab, Department of Bioscience and Bioengineering, Indian Institute of Technology (IIT), Guwahati under the supervision of Prof. Ajaikumar B. Kunnumakkara.  My primary interest lies in exploring the mechanistic cues of tumor pathophysiology in the early stage of transformation. I believe that cancer cells are both static and dynamic in the temporal planes, and intervening at the early stage will help circumvent the disease. The discovery of microRNAs has brought a paradigm shift in biological research, making it possible for us to focus on the molecular aspects of regulation in disease etiology. My research goals are to develop novel site-specific and effective treatment regimens using non-coding RNAs. 

I previously interned at the CSIR-Centre for Cellular and Molecular Biology (CCMB), India, centered around studying the mechanism of initiation and progression of colorectal cancer by using transgenic murine models. I was also a part of the team to understand the effect of DNA damaging drugs on anomalous EMT activation in the early stage of drug treatment, leading to drug resistance. The other projects I had worked on focused on the suppression of breast and colon cancer autochthonous models by using RNA interference and Nanotechnology. After completing my graduation, I joined CCMB as a project trainee to develop and validate the biomarkers for different sub-types (benign, borderline, fibro adenocarcinoma, malignant) of the phyllode tumors using global proteomic analysis, IHCs, and IFs. Apart from this project, I was also working on validating plausible microRNAs for breast cancer diagnosis.

Education

2017

 

Bachelors of Technolgy (BTech)

Specialization- Biotechnology 

Vellore Institute of Technology (VIT), Vellore 

Grades- 9.32/ 10

2019

 

Master's of Technolgy (MTech)

Specialization- Biotechnology 

Maulana Azad National Institute of Technology (NIT), Bhopal

Grades- 9.58/ 10

2020

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Pursuing Doctor of Philosophy (DPhil)

Indian Institute of Technology (IIT) Guwahati

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Experience

2016-2017

Center for Biomaterials, Cellular and Molecular Thernaostic, VIT University

Undergraduate Dissertation Research 

Probable role of non-extracellular vesicles in colorectal cancer metastasis to kidney: an in vitro cell line-based study and image analysis

2018-2019

CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad

Graduate Dissertation Research 

Genotyping, Characterization and Pre-clinical Studies of transgenic murine models of Colorectal Cancer 

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Identification  and Characterization of biomarkers for different sub-types (benign, borderline, fibro adenocarcinoma,
malignant) present in phyllode tumors

2019-2020

CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad

Project Trainee

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Laboratory Skills 

In vivo Techniques

Mice Handling, Breeding, Injections- Intraperitoneal, Intratumoral, Oral guvaging, Cardiac Perfusion,
Anesthetize, Histology, Tissue Processing, Sectioning

Immunological Assays

Immunoblots, ELISA, Immunocytochemistry, Immunohistochemistry, Rocket Electrophoresis

Animal Cell Culture

Subculturing, MTT assay, Invasion assay, Scratch assay, FACS, Colony formation assay, Transfection

Microbial Techniques

Plating, Streaking, Transformation, Cloning, Staining, Restriction Digestion

Molecular Biology Techniques

DNA, RNA and Protein Isolation, Plasmid Isolation, PCR, RT-PCR, Agarose Gel Electrophoresis

Computational Techniques

RNA seq analysis, Primer Design, BioEdit, ClustalW, MEGA6, Phylognetics, Autodock, miRNA Target Predcition

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Conference Presenations 

Feburary 2023

December 2021

March 2019

October 2016

Poster Presentation | Prime Minister's Research Fellows Annual Symposium 2023, Indian Institute of Technology (IIT) Madras 

Development of miRNA mimics targeting Tumor necrosis factor-α induced protein eight (TNFAIP8/TIPE) in oral cancer

Oral Presentation | 2nd International Conference on Cancer Metastasis, Austria

Combinatorial therapy using RNAi and curcumin nano- architectures regress tumors in breast and colon cancer models

Oral and Poster Presentation | International Conference on Advances in Chemical Biology and Biologics (ICACB 2019), CSIR- INDIAN INSTITUTE OF CHEMICAL TECHNOLOGY, Hyderabad
Activation of Epithelial-Mesenchymal Transition (EMT) during early drug response inhibits apoptotic progression by stimulating survival pathways in the colon carcinoma model

Poster Presentation | International Conference on Nanoscience and Nanotechnology (ICNAN’16), VELLORE INSTITUTE OF TECHNOLOGY (VIT), Vellore 

Use of Membrane filter for isolation of Nanoscopic Extracellular Vesicles from cultured A549 lung cancer cell : A feasibility study

Honours and Awards 

2021:  ”Certificate of Appreciation” by European Accreditation Council for Continuing Medical Education (EACCME®) for presentation at 2nd International Conference on Cancer Metastasis, Austria

2021: Recipient of Prime Minister's Research Fellow (PMRF) for carrying out the vision of research and development through innovation, Ministry of Education (MOE), Govt. of India
2020:  ”Certificate of Appreciation" for contribution as an “Instructor” in the Winter Research Observership Programme for Medical Students (MBBS) at CSIR- Centre for Cellular and Molecular Biology, Hyderabad under
CSIR Integrated Skill Initiative
2019:  Best Oral Presentation in International Conference on Advances in Chemical Biology and Biologics (ICACB 2019), by Royal Society of Chemistry, CSIR-Indian Institute of Chemical Technology, Hyderabad
2019:  Merit Scholar, First Rank in Department of Biological Science and Engineering, MANIT, Bhopal
2019:  Recipient of GATE-MHRD fellowship from the Ministry of Human Resource Development, Govt. of India
2014: Merit Certificate, Best academic performance in Vellore Institute of Technology (VIT)

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Referees  

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Ajaikumar B Kunnumakkara

Professor, Indian Instittute of Technology (IIT) Guwahati

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Lekha Dinesh Kumar

Senior Principal Scientist, CSIR- Centre for Cellular and Molecular Biology, Hyderabad

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Anindya Goswami

Senior Principal Scientist, CSIR- Indian Institute of Integrative Medicine, Jammu

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Hitesh Kulhari 

Assistant Professor and DST INSPIRE Faculty, Central University of Gujarat, Gandhinagar

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